The INI has a new website!

This is a legacy webpage. Please visit the new site to ensure you are seeing up to date information.

Skip to content



Co-operative roles for DNA supercoiling and nucleoid-associated proteins in the regulation of bacterial transcription

Dorman, C (Trinity College Dublin, Ireland)
Wednesday 05 September 2012, 09:10-09:50

Seminar Room 1, Newton Institute


DNA supercoiling and nucleoid-associated proteins (NAPs) contribute to the regulation of transcription of many bacterial genes. The horizontally-acquired Salmonella pathogenicity island (SPI) genes respond positively to DNA relaxation, to the Fis and H-NS nucleoid-associated proteins and to the OmpR global regulatory protein. The ompR gene is autoregulated and responds positively to DNA relaxation. Binding of the Fis and OmpR proteins to DNA in the SPI-1 and SPI-2 islands and at the ompR gene promoter is differentially sensitive to the topological state of the DNA while H-NS binds regardless of the topological state of the DNA. These data illustrate the overlapping and complex nature of NAP and DNA topological contributions to transcription control in bacteria. They also show that these properties are shared by the core and the horizontally-acquired components of the bacterial genome.


The video for this talk should appear here if JavaScript is enabled.
If it doesn't, something may have gone wrong with our embedded player.
We'll get it fixed as soon as possible.

Back to top ∧